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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Microbiol. 2021 Jan 11;6(2):221–233. doi: 10.1038/s41564-020-00823-6

Extended Data Fig. 3. Cryo-EM workflow for PorLM.

Extended Data Fig. 3

a, Image processing workflow for PorLM. Scale bar is 100 Å.

b, Fourier Shell Correlation (FSC) plot for PorLM Red, phase randomised; Green, unmasked; Blue, masked; Black, corrected.

c, Local resolution histogram of PorLM volume

d, Particle Orientation Distributions from final refined volume. Rotation (phi) is plotted along the x axis and tilt (theta) on the y axis. The area of the dot at the angular position is proportional to the number of particles assigned to that view.