Figure 6. Extended Data Figure 6.

Specificity of ribozyme for the target tRNA. a. Secondary structure schemes of six E.coli tRNAs, with very similar TΨC-stem-loop sequences (drawn without natural modifications). Target tRNA^Asp in top left corner, with A58 indicated in blue. The nucleotides complementary to the binding arms of MTR1-tRNA^Asp-A58 (Rz23) are shown in bold. The purple nucleotides indicate mismatched positions with the binding arms. b. Full gel image of primer extension analysis on total E.coli tRNA with six different primers, shown in Fig. 3c. Primer extension analyses were repeated twice.