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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nature. 2020 Oct 28;587(7835):663–667. doi: 10.1038/s41586-020-2854-z

Figure 2. Extended Data Figure 2.

Figure 2

Activity of methyltransferase ribozymes: Examination of mutations in the stem-loop. a. 3'-Fluorescein-labeled R10a tested with 100 μM m6G, b. 5'-Fluorescein-labeled R1 tested with 100 μM BG-NH2. ON = over night (23 h) c. Kinetics of CA13 (Rz1/Rz1s)-catalysed alkylation of R1 using BG-NH2 cofactor. Fraction modified is shown as mean ± stdev (n = 3), and fit to a monexponential model (Y = Ymax (1 – e–kt), blue), or a biexponential model (Y = Ymax (a (1 – e–k1t) + (1 – a) (1 – e–k2t)), black). d. Dependence of MTR1 methylation yield on Mg2+ concentration, reactions performed with 100 μM m6G (on R2 with Rz3) at 37°C. Individual data points are shown as empty symbols (n = 2 for 5 h and 23 h, n = 3 for 7 h timpoints), and mean is depicted as filled symbol.