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RNA-catalyzed methylation of tRNAs. a. tRNA sequences studied. b. Synthetic fragments and corresponding ribozymes. c. Exemplary gel images for kinetic analysis of MTR1-catalysed fragment methylation showing quantitative formation of m1A. d. Exemplary HR-ESI MS of isolated methylated R.norvegicus RNA fragment. e. Full gel images of primer extension analyses shown in Fig 3. Representative gel images of three independent experiments with similar results.
