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. Author manuscript; available in PMC: 2021 Feb 22.
Published in final edited form as: Nat Neurosci. 2016 Jun 13;19(8):995–8. doi: 10.1038/nn.4325

Figure 2. Blocking transport within the lysosomal pathway of microglia results in intracellular myelin storage and age-associated immune activation.

Figure 2

(a) Confocal image shows colocalization (white arrows) of MBP (green) with Iba1-positive microglia (red). Scale bars, 30 μm (left); 2 μm (right). Quantification of MBP-immunoreactive puncta in microglia of the white matter in 9-month-old control and Rab7 ΔMG mice (n = 3 mice per group; mean ± s.d.; **P = 0.0058, t = 5.376, d.f. = 4; Student’s two-tailed t test). (b) Colocalization of myelin fragments (FluoroMyelin (FM), green) with lipofuscin (LF, gray) within microglia in 12-month-old mice (n = 3 mice per group; mean ± s.d.; ***P < 0.0001, t = 21.265; d.f. = 4; Student’s two-tailed t test). Scale bars, 2 μm. (c) Quantification of lipofuscin volume in μm3 in Rab7 ΔMG mice as compared to controls (n = 3 mice per group, mean ± s.d.; two-way ANOVA; genotype effect, **P = 0.0033, F = 17.09; followed by Bonferroni’s post hoc test; *P < 0.05, ***P = 0.0006, t = 7.145). Each dot represents the mean value of 40 cells. (d) Visualization and quantification of MHC-II-positive microglia in Rab7 ΔMG mice and controls (n = 3 mice per group; mean ± s.d.; two-way ANOVA; genotype effect, ***P = 0.0007, F = 19.70; followed by Bonferroni’s post hoc test; *P = 0.0412, t = 2.893, **P = 0.0081, t = 4.782). n.s., nonsignificant (P = 0.3221). Scale bar, 50 μm. In b and d each dot represents the mean value of 3 brain slices per mouse. All images are representative of three independent experiments.