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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Nature. 2020 Oct 14;587(7833):303–308. doi: 10.1038/s41586-020-2815-6

Extended Data Figure 2. TERRA associates preferentially with short telomeres.

Extended Data Figure 2

a, TRF analysis of HeLa clones used for transient expression of TERRA b, d, TERRA expression levels measured by RT-qPCR and compared to the levels of TERRA in the clone with the shortest telomeres. n=3 biologically independent experiments, data are mean ± s.d. c, TRF analysis of HeLa clones expressing transgenic TERRA from the AAVS1 locus. e, HeLa clones of different telomere length were transiently transfected with the PP7-15q TERRA construct and co-localization was assessed by IF-FISH. The percentage of TERRA foci that co-localized with telomeres was plotted as a function of telomere length. White arrows indicate co-localization of PP7 foci with the telomeric signal. Random colocalization events (~3%) were not subtracted. f, Co-localization events as in e but for PP7-15q TERRA expressed from the AAVS locus on chromosome 19. n=3 biologically independent experiments, data are mean ± s.d. Significant differences are indicated. Two-tailed unpaired t test was used to calculate the P values. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P<0.0001).