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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Nature. 2020 Oct 14;587(7833):303–308. doi: 10.1038/s41586-020-2815-6

Extended Data Figure 3. Depletion of factors regulating TERRA trafficking.

Extended Data Figure 3

a, Timeline of transfections and cell harvest. mRNA levels were determined by RT-qPCR for both long and short telomere cell lines. b, For the second replicate of the siRNA screen the functional level of depletion was also evaluated on Western blots. Vinculin and hnRNPA1 were used as loading controls. c, Number of TERRA foci per cell were plotted for each depleted factor. n=2 biologically independent experiments, ≥ 40 nuclei were analyzed per condition. d, Quantification of endogenous TERRA stemming from chromosome ends 15q, 13q and 10q upon depletion of the indicated factors relative to the negative control (siCONTROL). n=2 biologically independent experiments.