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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Nature. 2020 Oct 14;587(7833):303–308. doi: 10.1038/s41586-020-2815-6

Extended Data Table 1. Oligonucleotides used in this study.

Primers used for Chimeric TERRA constructs
Name Forward Reverse
Amplification of PP7 stem loops CCGCGGCCCCGAATTCTATCGATACTCGAGATCCTA GCTGACTAGAGGATCCACACGCGTTCTCGATAATGAA
Amplification of TTAGGG repeats TTAGGGTTAGGGTTAGGGTTAGGGTTAGGG CCCTAACCCTAACCCTAACCCTAACCCTAA
Amplification of 15q subtelomere GAACGCGTGTGGATCATTCTCCTCAGGTCAGACCCG CTAACCCTAAGGATCCTAACCGTGACCCTGACCCCG
Amplification of Xq subtelomere GAACGCGTGTGGATCCCGAGTTGCGTTCTCG CTAACCCTAAGGATCGCACATGAGGAATGTGGGTG
Amplificiation of AAVS1 left homology arm TGACCGGTTGCTTTCTCTGACCAGCATTC GTGTTAACCACTGTGGGGTGGAGGGGAC
Amplificiation of AAVS1 right homology arm CGGATATCACTAGGGACAGGATTGGT GTGATATCCTGTAGGAAGGGGCAGGAGA
Primers used for RT-qPCR
Name Forward Reverse
15qTERRA CAGCGAGATTCTCCCAAGCTAAG AACCCTAACCACATGAGCAACG
GAPDH AGCCACATCGCTCAGACAC GCCCAATACGACCAAATCC
TRF1 CTTGCCAGTTGAGAACGATATACA CATCAGGGCTGATTCCAAGG
TRF2 GGGTTATGCAGTGTCTGTCGCG CAGTGGTGTGAGCTCAGCCT
POT1 CCAAGCTCTGGATCAGTATCATT CATAGTGGTGTCCTCTCAAATAC
SMG1 CCAAGCACCGTTCCAGGAACTG CTCTCTTGCACCGCTTTCCCAG
UPF1 CGCAGGGCTACATCTCCATGAG CTCGTCACCAAGGTAACTGTCCTG
UPF2 GGCTGAGTCTGCAGACACAATGC GCAGCAAGTTGAGAGGACATGGG
RNaseHI GGCCAGGCCATCCTTTAAATGTAGG GCTTGTTCAATGGCTTTGCAGGC
RPA2 GCAGGGAACTTTGGTGGGAATAGC CCCTTCAGGTCTTGGACAAGCC
RAD51 GAGGAAAGGAAGAGGGGAAACCAG CCCACTCCATCTGCATTAATGGCG
1q subtelomere CAGCGTCGCAACTCAAATG CCCTCACCCTCCATGAGTAATA
10q subtelomere GCATTCCTAATGCACACATGAC TACCCGAACCTGAACCCTAA
13q subtelomere GCACTTGAACCCTGCAATACAG CCTGCGCACCGAGATTCT
15q subtelomere AACCCTAACCACATGAGCAACG GCTGCATTAAAGGGTCCAGT
siRNAs
Gene name Catalog number siRNA sequences
siControl D-001206-13-20 UAGCGACUAAACACAUCAA, UAAGGCUAUGAAGAGAUAC,
AUGUAUUGGCCUGUAUUAG, AUGAACGUGAAUUGCUCAA
SiTRF1 M-010542-02 CAAGAUAAACCUAGUGGUA, GGUGAUCCAAAUUCUCAUA,
GGAAACUGGUCUAAAAUAC, GCCAGUUGAGAACGAUAUA
siTRF2 M-003546-00 GAAGUGGACUGUAGAAGAA, GGAAGCUGCUGUCAUUAUU,
GGAAUCAGCUAUCAAUGUG, GAAGACAGUACAACCAAUA
SiPOT1 M-004205-01 AGAAAGAUGUCAACAGCUA, GAGGCAAAGAAUCGAAAUA,
CAGGAGUACUAGAAGCCUA, UGCAAGAUCUCCACGUUAA
SiSMG1 M-005033-01 GUGAAGAUGUUCCCUAUGA, GAGGUUAGCUGCGGAAAGA,
GGUCAGACAUCCACCAGAA, UAACUUGGCUCAGCUGUAU
SiUPF1 M-011763-01 GCUCCUACCUGGUGCAGUA, UCAAGGUCCCUGAUAAUUA,
GGAAGUCGACCUCCUUUGA, CAAGAUAACAUCACUGUCA
siUPF2 M-012993-01 GGAACGAGAAUUCUUAAUA, GCAUGUACCUUGUGUAGAA,
GAAGAUAUUCGAUUAGGAA, GGUCUAGAGAGUUGCGAAU
siRNaseH1 M-012595-00 GCGCAGAGCCGUAUGCAAA, GAGCUAAACAAUCGGAAGA,
GCCAGGCCAUCCUUUAAAU, GACAUUCAGUGGAUGCAUG
siRPA2 M-017058-00 GAUCAAUGCACACAUGGUA, CAAAAUAGAUGACAUGACA,
GAGUGAAGCAGGGAACUUU, GUGGAACAGUGGAUUCGAA
SiRAD51 M-003530-04 GAAGCUAUGUUCGCCAUUA, GCAGUGAUGUCCUGGAUAA,
CCAACGAUGUGAAGAAAUU, AAGCUAUGUUCGCCAUUAA
siBRCA2 M-003462-01 GAAACGGACUUGCUAUUUA, GUAAAGAAAUGCAGAAUUC,
GGUAUCAGAUGCUUCAUUA. GAAGAAUGCAGGUUUAAUA