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. Author manuscript; available in PMC: 2021 Feb 22.
Published in final edited form as: J Biotechnol. 2018 Dec 11;291:52–60. doi: 10.1016/j.jbiotec.2018.12.001

Fig. 4.

Fig. 4

Activity of EziG3-AsR in kinetic resolution of rac-α-MBA when applying higher substrate loadings. Conversion to acetophenone (striped bars) and TON (white bars) for the reaction catalyzed by the immobilized enzyme. Error bars display standard deviation over three experiments. Reaction conditions: EziG3-AsR (10mg, 17% enzyme loading, w w-1); rac-α-MBA (concentration varied); sodium pyruvate (0.5 equiv.); DMSO (10%, v v-1); PLP (25 pM); HEPES buffer (0.5 mL reaction volume, 250mM, pH 7); 30 °C, thermomixer (750 rpm); 15 min.