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. Author manuscript; available in PMC: 2021 Aug 15.
Published in final edited form as: Nat Metab. 2021 Feb 15;3(2):258–273. doi: 10.1038/s42255-021-00344-4

Extended Data Fig. 5. PPGNTS neurons are necessary for oxytocin-induced eating suppression.

Extended Data Fig. 5

(a-b) Food intake and bodyweight change over 1 day in eGFP and DTA mice (n=5 (DTA) / 7 (eGFP) animals) administered oxytocin (0.4 mg/kg, i.p.), 2-way mixed-model ANOVA: a) Drug F(1,10)=0.00474, p=0.947; b) Drug F(1,10)=0.0989, p=0.760.

(c-d) Photomicrographs of coronal NTS sections from PPG-Cre:GCaMP3 mice injected with eGFP control virus (c) or DTA virus (d). Note the complete absence of green (GCaMP3-expressing, amplified by immunostaining against the GFP antigen) PPGNTS neurons in DTA-ablated tissue, and the extent of viral spread as demonstrated by constitutive expression of mCherry (photomicrographs representative of independent experiments from 7/5 animals). Distance in mm posterior to Bregma in bottom left, cc: central canal. Scale=100μm.

All data presented as mean ± SEM.