Extended Data Fig. 7. Liraglutide and semaglutide suppress eating independently of PPG^NTS neurons.

(a-e) Cumulative food intake by virus at 1,2,4,6 and 21hr in eGFP and DTA mice (n=8 (DTA) / 7 (eGFP) animals for analyses presented in a-j) administered liraglutide (200 μg/kg, s.c.), 2-way mixed-model ANOVA: a) Drug F_(1,13)=0.246, p=0.628; b) Drug F_(1,13)=2.108, p=0.170; c) Drug F_(1,13)=37.44, p<0.0001, Virus F_(1,13)=0.836, p=0.377; d) Drug F_(1,13)=75.09, p<0.0001, Virus F_(1,13)=1.877, p=0.194; e) Drug F_(1,13)=154.9, p<0.0001, Virus F_(1,13)=1.272, p=0.280.

(f-j) Cumulative food intake by virus at 1,2,4,6 and 21hr in eGFP and DTA mice administered semaglutide (60 μg/kg, s.c.), 2-way mixed-model ANOVA: f) Drug F_(1,13)=1.965, p=0.184; g) Drug F_(1,13)=17.1, p=0.0012; Virus F_(1,13)=0.630, p=0.442; h) Drug F_(1,13)=82.49, p<0.0001, Virus F_(1,13)=0.332, p=0.574; i) Drug F_(1,13)=98.21, p<0.0001, Virus F_(1,13)=0.840, p=0.376; j) Drug F_(1,13)=126.1, p<0.0001, Virus F_(1,13)=3.42, p=0.0873.

(k-m) Representative photomicrographs of cFos immunoreactivity (cFos-ir) in arcuate nucleus of the hypothalamus (ARC) 4 hours after vehicle (VEH, n=4 animals) or semaglutide (SEMA, 60 μg/kg, s.c., n=4 animals) administration, and total cFos count, unpaired 1-tailed t-test: m) t₍₆₎=2.614, p=0.020. Scale=100μm.

(n-p) Representative photomicrographs of cFos-ir in paraventricular nucleus of the hypothalamus (PVN) 4 hours after vehicle or semaglutide administration (n=4 / 4 animals), and total cFos count, unpaired 1-tailed t-test: p) t₍₆₎=5.109, p=0.0011. Scale=100μm.

(q-t) Representative photomicrographs of cFos-ir in dorsal lateral and external lateral subdivisions of the parabrachial nucleus (dlPBN / elPBN) 4 hours after vehicle or semaglutide administration (n=3 / 4 animals), and total cFos count, unpaired 1-tailed t-tests: s) t₍₅₎=1.693, p=0.0756; t) t₍₅₎=3.57, p=0.0080. Semaglutide did not increase cFos-ir in the medial PBN, t₍₅₎=0.435, p=0.341. Scale=100μm.

All data presented as mean ± SEM.