(a) Experimental model and paradigm for ad libitum pellet eating from FED in PPGNTS- hM3Dq mice. n=7 animals for analyses presented in b-d.
(b) Daily food intake during 48h test, 2-way within-subjects ANOVA: Drug × Day F(1,6)=14.52, p=0.0089.
(c) Cumulative hourly food intake over two days, 2-way within-subjects ANOVA: Drug × Time F(48,288)=6.481, p<0.0001.
(d) 24h and 48h bodyweight change, 2-way within-subjects ANOVA: Drug F(1,6)=10.41, p=0.018.
(e) Experimental model and paradigm for BSS analysis in 18h fasted PPGNTS-hM3Dq mice. n=7 animals for analyses presented in f-k.
(f-g) Behavioural satiety sequences following saline and CNO injections. Satiation point/satiety onset (when duration inactive exceeds eating) shown by dotted lines.
(h-k) Quantitative analysis of hM3Dq effect on BSS behaviours, 2-way with-subjects ANOVA: h) Drug × Time F(7,42)=5.673, p=0.0001; i) Drug F(1,6)=5.261, p=0.0616; j) Drug F(1,6)=12.48, p=0.0123; k) Drug F(1,6)=4.028, p=0.0915.
All data presented as mean ± SEM.