Antibodies & Viruses |
α-DsRed rabbit pAb (1:500 IF) |
Takara Bio |
Cat #632496, RRID:AB_10013483 |
α-mCherry rabbit pAb (1:500 IF) |
Abcam |
Cat# ab167453, RRID:AB_2571870 |
α-GFP chicken pAb (1:1000 IF) |
Abcam |
Cat #13970, RRID:AB_300798 |
α-cFos (9F6) rabbit mAb (1:1000 IF / 1:10,000 IHC) |
Cell Signaling Technology |
Cat# 2250, RRID:AB_2247211 |
α-cFos rabbit pAb (1:500 IF) |
Millipore |
Cat# ABE457, RRID:AB_2631318 |
α-tyrosine hydroxylase rabbit pAb (1:1000 IF) |
Millipore |
Cat# AB152, RRID:AB_390204 |
α-rabbit IgG biotinylated goat pAb (1:500 IHC) |
Vector Laboratories |
Cat# BA-1000, RRID:AB_2313606 |
Alexa Fluor 488 goat α-chicken (1:500 IF) |
Thermo Fisher Scientific |
Cat# A-11039, RRID:AB_2534096 |
Alexa Fluor 488 goat α-rabbit (1:500 IF) |
Thermo Fisher Scientific |
Cat# A-11008, RRID:AB_143165 |
Alexa Fluor 568 donkey α-rabbit (1:500 IF) |
Thermo Fisher Scientific |
Cat# A-10042, RRID:AB_2534017 |
Alexa Flour 647 donkey α-rabbit (1:500 IF) |
Thermo Fisher Scientific |
Cat# A-31573, RRID:AB_2536183 |
AAV8-EF1a-mCherry-DIO-DTA (3.3×1012) |
UNC Vector Core, NC |
Gift from Naoshige Uchida, RRID:Addgene_5853664
|
AAV8-hSyn1-DIO-mCherry (9.0×1012) |
Viral Vector Facility, ETH Zurich |
Cat# v116–8, Gift from Bryan Roth, RRID:Addgene_50459 |
AAV8-hSyn1-DIO-eGFP (6.3×1012) |
Viral Vector Facility, ETH Zurich |
Cat# v115–8, Gift from Bryan Roth, RRID:Addgene_50457 |
AAV2-hSyn1-DIO-hM4Di:mCherry (6.4×1012) |
UNC Vector Core, NC |
Lot AV4500F |
AAV8- hSyn1-DIO-hM3Dq:mCherry (4.5×1012) |
Viral Vector Facility, ETH Zurich |
Cat# v89–8, Gift from Bryan Roth, RRID:Addgene_4436165
|
AAV5- hSyn1-DIO-hM3Dq:mCherry (≥7×1012) |
Addgene Viral Service |
Cat# 44361-AAV5, Gift from Bryan Roth, RRID:Addgene_4436165
|
AAV9- hSyn1-DIO-hM3Dq:mCherry (≥7×1012) |
Addgene Viral Service |
Cat# 44361-AAV9, Gift from Bryan Roth, RRID:Addgene_4436165
|
AAV5-EF1a-DIO-hChR2(H134R):mCherry (5.7×1012) |
UNC Vector Core, NC |
Gift from Karl Deisseroth, RRID:Addgene_20297 |
AAV9-EF1a-DIO-hChR2(H134R):eYFP (≥1×1013) |
Addgene Viral Service |
Cat# 20298-AAV9, Gift from Karl Deisseroth, RRID:Addgene_20297 |
AAV-PHP.S-CAG-DIO-tdTomato (≥1×1013) |
Addgene Viral Service |
Cat# 28306-PHP.S, Gift from Edward Boyden, RRID:Addgene_28306 |
AAV5-EF1a-FLEX-TVA:mCherry (5.6×108) |
Stanford Gene Vector and Virus Core |
Cat# GVC-AAV-67, Gift from Karl Deisseroth66
|
AAV8/733-CAG-FLEX-RabiesG (2.13×1012) |
Stanford Gene Vector and Virus Core |
Cat# GVC-AAV-5966
|
(EnvA)-RV-ΔG-GFP (2×108) |
Kevin Beier, UC Irvine |
67 |
Drugs, Chemicals & Assays |
Clozapine N-Oxide (CNO) |
Hello Bio |
Cat# HB1807 |
Clozapine N-Oxide (CNO) |
Enzo Life Sciences |
Cat# BML-NS105 |
Oxytocin |
Tocris |
Cat# 1910 |
Liraglutide |
Novo Nordisk A/S |
Batch GP52108, Gift from Lotte Bjerre Knudsen |
Semaglutide |
Novo Nordisk A/S |
Batch GV40057, Gift from Lotte Bjerre Knudsen |
Ensure Plus (vanilla) |
Abbott |
Cat# ENS100V |
Purified dustless precision pellets (20mg) |
Bio-Serv |
Cat# F0071 |
Vectastain Elite ABC-Peroxidase Kit |
Vector Laboratories |
Cat# PK-7100, RRID:AB_2336827 |
RNAscope Multiplex Fluorescent Kit v2 |
Advanced Cell Diagnostics |
Cat# 323100 |
RNAscope target probe for mouse Glp1r
|
Advanced Cell Diagnostics |
Cat# 418851-C2 |
RNAscope target probe for mouse Oxtr
|
Advanced Cell Diagnostics |
Cat# 402651-C3 |
RNAscope positive control probe (mouse Ubc) |
Advanced Cell Diagnostics |
Cat# 310771-C2/3 |
RNAscope negative control probe (mouse DapB) |
Advanced Cell Diagnostics |
Cat# 310043-C2/3 |
Mice |
mGlu-Cre/tdRFP (referred to here as PPG-Cre:tdRFP) |
Frank Reimann, University of Cambridge53
|
mGlu-Cre/GCaMP3 (referred to here as PPG-Cre:GCaMP3) |
Frank Reimann, University of Cambridge42
|
mGlu-YFP (referred to here as PPG-YFP) |
Frank Reimann, University of Cambridge52
|
Glp1r-Cre/tdRFP (referred to here as GLP-1R-Cre:tdRFP) |
Frank Reimann, University of Cambridge54
|
mGlu-YFP × Glp1r-Cre/tdRFP (referred to here as GLP-1R-Cre × PPG-YFP) |
Strains from Frank Reimann crossed at UCL13
|