Table 1.
Reagents and Resources
| Antibodies & Viruses | ||
| α-DsRed rabbit pAb (1:500 IF) | Takara Bio | Cat #632496, RRID:AB_10013483 |
| α-mCherry rabbit pAb (1:500 IF) | Abcam | Cat# ab167453, RRID:AB_2571870 |
| α-GFP chicken pAb (1:1000 IF) | Abcam | Cat #13970, RRID:AB_300798 |
| α-cFos (9F6) rabbit mAb (1:1000 IF / 1:10,000 IHC) | Cell Signaling Technology | Cat# 2250, RRID:AB_2247211 |
| α-cFos rabbit pAb (1:500 IF) | Millipore | Cat# ABE457, RRID:AB_2631318 |
| α-tyrosine hydroxylase rabbit pAb (1:1000 IF) | Millipore | Cat# AB152, RRID:AB_390204 |
| α-rabbit IgG biotinylated goat pAb (1:500 IHC) | Vector Laboratories | Cat# BA-1000, RRID:AB_2313606 |
| Alexa Fluor 488 goat α-chicken (1:500 IF) | Thermo Fisher Scientific | Cat# A-11039, RRID:AB_2534096 |
| Alexa Fluor 488 goat α-rabbit (1:500 IF) | Thermo Fisher Scientific | Cat# A-11008, RRID:AB_143165 |
| Alexa Fluor 568 donkey α-rabbit (1:500 IF) | Thermo Fisher Scientific | Cat# A-10042, RRID:AB_2534017 |
| Alexa Flour 647 donkey α-rabbit (1:500 IF) | Thermo Fisher Scientific | Cat# A-31573, RRID:AB_2536183 |
| AAV8-EF1a-mCherry-DIO-DTA (3.3×1012) | UNC Vector Core, NC | Gift from Naoshige Uchida, RRID:Addgene_5853664 |
| AAV8-hSyn1-DIO-mCherry (9.0×1012) | Viral Vector Facility, ETH Zurich | Cat# v116–8, Gift from Bryan Roth, RRID:Addgene_50459 |
| AAV8-hSyn1-DIO-eGFP (6.3×1012) | Viral Vector Facility, ETH Zurich | Cat# v115–8, Gift from Bryan Roth, RRID:Addgene_50457 |
| AAV2-hSyn1-DIO-hM4Di:mCherry (6.4×1012) | UNC Vector Core, NC | Lot AV4500F |
| AAV8- hSyn1-DIO-hM3Dq:mCherry (4.5×1012) | Viral Vector Facility, ETH Zurich | Cat# v89–8, Gift from Bryan Roth, RRID:Addgene_4436165 |
| AAV5- hSyn1-DIO-hM3Dq:mCherry (≥7×1012) | Addgene Viral Service | Cat# 44361-AAV5, Gift from Bryan Roth, RRID:Addgene_4436165 |
| AAV9- hSyn1-DIO-hM3Dq:mCherry (≥7×1012) | Addgene Viral Service | Cat# 44361-AAV9, Gift from Bryan Roth, RRID:Addgene_4436165 |
| AAV5-EF1a-DIO-hChR2(H134R):mCherry (5.7×1012) | UNC Vector Core, NC | Gift from Karl Deisseroth, RRID:Addgene_20297 |
| AAV9-EF1a-DIO-hChR2(H134R):eYFP (≥1×1013) | Addgene Viral Service | Cat# 20298-AAV9, Gift from Karl Deisseroth, RRID:Addgene_20297 |
| AAV-PHP.S-CAG-DIO-tdTomato (≥1×1013) | Addgene Viral Service | Cat# 28306-PHP.S, Gift from Edward Boyden, RRID:Addgene_28306 |
| AAV5-EF1a-FLEX-TVA:mCherry (5.6×108) | Stanford Gene Vector and Virus Core | Cat# GVC-AAV-67, Gift from Karl Deisseroth66 |
| AAV8/733-CAG-FLEX-RabiesG (2.13×1012) | Stanford Gene Vector and Virus Core | Cat# GVC-AAV-5966 |
| (EnvA)-RV-ΔG-GFP (2×108) | Kevin Beier, UC Irvine | 67 |
| Drugs, Chemicals & Assays | ||
| Clozapine N-Oxide (CNO) | Hello Bio | Cat# HB1807 |
| Clozapine N-Oxide (CNO) | Enzo Life Sciences | Cat# BML-NS105 |
| Oxytocin | Tocris | Cat# 1910 |
| Liraglutide | Novo Nordisk A/S | Batch GP52108, Gift from Lotte Bjerre Knudsen |
| Semaglutide | Novo Nordisk A/S | Batch GV40057, Gift from Lotte Bjerre Knudsen |
| Ensure Plus (vanilla) | Abbott | Cat# ENS100V |
| Purified dustless precision pellets (20mg) | Bio-Serv | Cat# F0071 |
| Vectastain Elite ABC-Peroxidase Kit | Vector Laboratories | Cat# PK-7100, RRID:AB_2336827 |
| RNAscope Multiplex Fluorescent Kit v2 | Advanced Cell Diagnostics | Cat# 323100 |
| RNAscope target probe for mouse Glp1r | Advanced Cell Diagnostics | Cat# 418851-C2 |
| RNAscope target probe for mouse Oxtr | Advanced Cell Diagnostics | Cat# 402651-C3 |
| RNAscope positive control probe (mouse Ubc) | Advanced Cell Diagnostics | Cat# 310771-C2/3 |
| RNAscope negative control probe (mouse DapB) | Advanced Cell Diagnostics | Cat# 310043-C2/3 |
| Mice | ||
| mGlu-Cre/tdRFP (referred to here as PPG-Cre:tdRFP) | Frank Reimann, University of Cambridge53 | |
| mGlu-Cre/GCaMP3 (referred to here as PPG-Cre:GCaMP3) | Frank Reimann, University of Cambridge42 | |
| mGlu-YFP (referred to here as PPG-YFP) | Frank Reimann, University of Cambridge52 | |
| Glp1r-Cre/tdRFP (referred to here as GLP-1R-Cre:tdRFP) | Frank Reimann, University of Cambridge54 | |
| mGlu-YFP × Glp1r-Cre/tdRFP (referred to here as GLP-1R-Cre × PPG-YFP) | Strains from Frank Reimann crossed at UCL13 | |