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. Author manuscript; available in PMC: 2021 Jul 25.
Published in final edited form as: Nat Cell Biol. 2021 Jan 25;23(2):136–146. doi: 10.1038/s41556-020-00627-0

Figure 5. Alleviating proteotoxic stress rescues the loser status.

Figure 5

(a-b) Apoptosis detection by cleaved caspase-3 staining (red) in competing wing discs containing RpS3 +/- cells (GFP-positive) and unlabeled wild type cells (GFP-negative) from larvae fed ethanol carrier (a) or 4 μM rapamycin (b). (c) Quantification of cell death at RpS3 +/- clone boundaries for the experiments in (a-b) (n=13 and 12 respectively, two-sided two sample Kolmgorov-Smirnov test). (d-e) GstD1-GFP signal (green) in RpS3 +/- wing discs fed EtOH control or 4μM Rapamycin, as indicated (d), and corresponding quantification (n=10 and 12 respectively, two-sided student’s t-test) (e). (f) p62 staining in RpS3 +/- wing discs expressing FOXO in P cells (labelled by the absence of Ci, magenta). (g-h) An RpS3 +/- wing disc harboring FOXO expressing clones (GFP-positive) and labelled with OPP (red) (g) with corresponding quantification in (h) (n=8, two-sided paired t-test). (i-j) Phospho-eIF2α staining (red) in RpS3 +/- wing discs expressing FOXO in P cells (i) and corresponding quantification (n=10, two-sided Wilcoxon signed-rank test. Due to low genetic frequency and the presence of an internal control, samples from multiple experiments were pooled together) (j). (k-l) Apoptosis detection by cleaved caspase-3 staining (red) in competing wild-type/RpS3 +/- mosaic wing discs without (k) or with (l) additional expression of dFOXO specifically in RpS3 +/- cells. (m) Quantification of cell death at RpS3 +/- clone boundaries for the experiments in (k-l) (n=8 and 10, respectively, two-sided two sample Kolmgorov-Smirnov test). For all micrographs, scale bars correspond to 50μm. For all quantifications provided, the horizontal line represents the mean and whiskers indicate 95% confidence intervals. All n numbers refer to the number of individual wing discs.