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. Author manuscript; available in PMC: 2021 Jul 18.
Published in final edited form as: Nat Microbiol. 2021 Jan 18;6(3):392–400. doi: 10.1038/s41564-020-00844-1

Fig. 4. VSGsur and the loss of contact mutants have indistinguishable endocytic kinetics.

Fig. 4

(a) Alexa 488-dextran endocytosis by T. b. brucei 2T1 cells expressing a variety of different VSG genes. Due to technical limitations, each cell line could not be analyzed simultaneously within each experiment for each ligand. Therefore, VSG2 was used as a control in all experiments, allowing the calculation of the relative uptake rate for each cell line rate as compared to the uptake rate of each ligand observed by VSG2 expressing cells within each separate experiment. Each of these graphs therefore represent the combined experimental results from 2 separate experiments. Cell lines with a relative fluorescence intensity below 1 at a given time point have less efficient endocytic rates compared to VSG2 expressing cells, and vice versa. All graphs share the same Y axis. Error bars when present denote the range (two individual experimental replicates for each cell line at every time point). (b)-(d) Alexa 488-dextran (b), bodipy-LDL (c), and Alexa 488-transferrin (d) endocytosis by T. b. brucei 2T1 cells expressing VSGsur (black triangles), VSG2 (pink circles), VSGsur H122A (blue hexagons), or VSGsur N130A (green diamonds). All graphs depict arbitrary fluorescence units, determined by flow cytometry, on the Y axis. Each graph represents one of multiple biological replicates of each experiment, with n=2 individual experimental replicates for each time point.