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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Immunol. 2021 Jan 11;22(2):166–178. doi: 10.1038/s41590-020-00833-w

Extended Data Fig. 6. RORα binds Id2 and Nfil3 regulatory elements.

Extended Data Fig. 6

(a) Representative ATAC-seq tracks for thymic ETP, DN2, DN3, NKp and ILC2p around the Bcl11b locus. Tracks shown are representative of three independent experiments.

(b) Representative ATAC-seq tracks for thymic ETP, DN2, DN3, NKp and ILC2p and binding profiles of RORα-T2A and GATA-3-T2A in ILC2 in ILC2 purified from lymph nodes of Rora teal/teal, Gata3hCD2TR/+ or wild type mice, and expanded in vitro with IL-7 and IL-33 around the Id2 and Nfil3 locus. Tracks shown are representative of three independent experiments.

(c) A luciferase assay shows the activity (relative to empty vector) of a DNA fragment containing the RORα-binding site from the Id2-associated -143 kb regulatory region, in the presence of increasing concentrations (as indicated) of RORα, GATA-3 or both. Data are representative of three independent experiments; mean ± SEM; two-way ANOVA with Tukey post-hoc test.