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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: ACS Appl Mater Interfaces. 2021 Jan 28;13(5):6011–6022. doi: 10.1021/acsami.0c20607

Figure 4. In vitro immuno-biological characterization of LNP.

Figure 4

(A) Cell viability measured by MTT assay. (n=6) (B) Flow cytometry analysis if DC2.4 cells pulsed with single-dye or double-dye labeled LNP. (C) Confocal microscopy of DC2.4 cells pulsed with LNP. Scale bar represents 15 microns. (D) Dose-response innate activation measured by the RAW Blue reporter cell assay (n=3). (E) Flow cytometry analysis of DC2.4 cells pulsed with different concentration of antigen loaded LNP followed by immunostaining with the OVA-Kb antibody (n=3; t-test *: p<0.05).