Table 1. Composition and properties of LNP measured by dynamic light scattering (DLS) and electrophoretic mobility analysis.
high N/COOH - low PEG | low N/COOH - low PEG | low N/COOH - high PEG | |
---|---|---|---|
N/COOH ratio | 10:1 | 5:1 | 5:1 |
DIPADS (mol%) | 50 | 50 | 49 |
DOPE (mol%) | 10 | 10 | 10 |
cholesterol (mol%) | 38.5 | 38.5 | 38.5 |
DSG-PEG (mol%) | 1.5 | 1.5 | 2.5 |
size [nm] | / | / | 87 |
PDI | 0.3 | 0.43 | 0.2 |
zeta potential [mv] | -3 | -3 | -7 |