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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nature. 2020 Oct 28;587(7835):626–631. doi: 10.1038/s41586-020-2857-9

Extended Data Figure 7. Slc1a5 knockdown in SC impairs the recovery of the muscle from CTX-induced damage.

Extended Data Figure 7

a-d, Quantification of TUNEL+ cells (a), F4/80+ area (c) and representative images (b,d) respectively, in TA muscle 6 days post-CTX obtained from LSL-Cas9 x PAX7:Cre-ERT mice treated with an AAV8 vector encoding for Ctrl gRNA (Ctrl gRNA) or Slc1a5 gRNA (SLC1A5-KD) (n=4).

e,f, Quantification (e) and representative images (f) of EdU+ myonuclei in TA muscle 6 days post-CTX, upon in vivo genome editing of the Slc1a5 locus (SLC1A5-KD) specific in SC. EdU was given by i.p. injection at 24h, 48h and 72h after CTX injection (n=6).

a-d show representative values of 2 independent experiments, e-f show values of 1 experiment. Unpaired two-tailed t-test was everywhere applied; ns, not significant (P>0.05). Scale bars: 20 μm (b,d,f). Graphs show mean ± SEM.