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. Author manuscript; available in PMC: 2021 Apr 28.
Published in final edited form as: Nature. 2020 Oct 28;587(7835):626–631. doi: 10.1038/s41586-020-2857-9

Extended Data Figure 2. GLUD1 loss in macrophages does not alter either their recruitment or M1/M2/wound-healing gene expression patterns.

Extended Data Figure 2

a, Crystal-violet-stained bone-marrow derived macrophages (BMDMs), migrating towards CCL21, CCL2 or PBS (Uns) in glutamine (Q)-enriched or Q-reduced media (n=3).

b,c, Quantification (b) and representative images (c) of F4/80 staining in ear-sections with acetone (vehicle) or upon phorbol ester (TPA)-induced cutaneous rash, 3 days and 8 days after TPA applying (Vehicle n=4; TPA Day3 n=6,5 CTRL and Glud1ΔMo, respectively; TPA Day8 n=4).

d, Heatmap analysis of M1 and M2 macrophage gene expression in CD45+ F4/80+ macrophages sorted from TA muscles at baseline and 1 day post-CTX (n=4).

e, Heatmap analysis of wound healing gene expression in CD45+ F4/80+ macrophages sorted from TA muscles at baseline and 1 day post-CTX (n=4).

a-c experiments show representative values of 2 independent experiments, d-e show values from one single experiment. Unpaired two-tailed t-test was applied in b; ns, not significant (P>0.05). Scale bars: 50 μm (c). Graphs show mean ± SEM.