Extended Data Fig. 9. Conservation of the MeCP2 protein domains.

(a) Amino acid multi-sequence alignment (MAFFT E-INS-i mode) of the Methyl-CpG Binding domain (MBD) from MeCP2, MBD4 and invertebrate MECP2/MBD4 sequences. The black square highlights the MBD domain as defined by Pfam. The red triangles indicate positions mutated in the human MECP2 gene that cause Rett Syndrome phenotypes⁵². (b) Amino acid multi-sequence alignment of the Transcriptional Repression Domain (TRD) from MeCP2, MBD4 and the homologous region (C-terminal of the MBD) of invertebrate MBD4/MECP2 proteins. NID stands for the N-CoR/SMRT interacting amino acids. Additional black squares highlight the AT-hook domains. Alignment visualised using Geneious software.