Fig. 4. Pax5-mediated recruitment of PRC2 to the Wapl promoter facilitates V_H-DJ_H recombination.

a, Epigenetic landscape at the Wapl locus in Pax5-deficient progenitors and Pax5-expressing control pro-B-cells. Binding of Pax5, Ezh2 and Suz12 and the presence of H3K4me3 and H3K27me3 were determined by ChIP-seq. One of two experiments per genotype and antibody is shown. b, Selective loss of H3K27me3 at the Wapl promoter in Rag1 ^Cre/+ Eed ^fl/fl and Wapl ^ΔP1/ΔP1 pro-B-cells. One of two experiments per genotype is shown. c, Wapl mRNA expression in ex vivo sorted pro-B-cells from mice of the indicated genotypes, as shown by mean TPM values. The P value and SEM (c, top) were determined by DESeq2. d, VDJ-seq analysis of pro-B-cells from Rag1 ^Cre/+ Eed ^fl/fl or control mice. The V_H gene usage is shown as mean percentage of all VDJ_H rearrangement events with SEM. n, number of experiments.