Fig. 3.
Di-8-ANEPPS-labeled (10 μM) membranes (POPC 200 μM LUV) fluorescence difference spectra (λem = 603 nm). The spectra were obtained by subtracting the excitation spectrum before the addition of peptides from the excitation spectra after the peptides addition (15 μM). Before subtraction, the spectra were normalized to the integrated areas so that the difference spectra reflect only the spectral shifts. (A) T-1249, (B) T20 and (C) S peptides. The latter spectra have no meaningful difference among each other.