Table 2.

Primers used for the cloning of chicken cDNA.

Gene	Forward/reverse	Sequence (5′–3′)	Melting Temperature (°C)
ChPLA2- IB	forward	5′-ATGAGACTCTTGGCGTGCTTTTCTTG-3′	56
ChPLA2-IB	reverse	5′-GACAAGAAGAAATACTGCACCAGTTAA-3′	56
ChPLA2-IIA	forward	5′ GTGTGTGTGAATTCATGAACATAGCTCAGTTTGGTATC 3′	60
ChPLA2-IIA	reverse	5′ GTGTGTCTCGAGCTAGCAAGAGGGACGTGAGCC 3′	60
ChPLA2-IIE	forward	5′ GTGTGTGTGTGAATTCATGAGGAATCTCCTGCTCGCC 3′	60
ChPLA2-IIE	reverse	5′ GTGTGTGTTCTAGATCAGCACTGGAGCTTGCTGCC 3′	60
ChPLA2-V	forward	5′ GTGTGTGTGAATTCATGAGCCTCTGGCAGCTGCAGGAG 3′	55
ChPLA2-V	reverse	5′ GTGTGTCTCGAGTCACCTGCACTTGCACCTGGG 3′	55
ChPLA2-X	forward	5′ GTGTGTGTGAATTCATGGGAATTCTTGAATTAGCTGGA 3′	58
ChPLA2-X	reverse	5′ GTGTGTCTCGAGTTAATATCTACACAAAGGTTG 3′	58