Table 3.
Primers used for the qPCR analyzes (f) : forward; (r) : reverse.
| Primer name | Sequence (5′–3′) | PCR product size | Melting temperature; Temperature of fluorescence measurement; |
|---|---|---|---|
| ChPLA2-IB (f) | AAGACTCTTGGCGTGCTTTTCTTGCTG | 200 | 56;89 |
| ChPLA2-IB (r) | GCTTGACAGCACCTGTCCAGTTCATCCA | 200 | 56;89 |
| ChPLA2-IIA (f) | AAGCTCTGGTGCTCCTCGTTGCCTT | 200 | 60;90.5 |
| ChPLA2-IIA (r) | CAGTCGTGGGCATGGCAGCACCAGTCG | 200 | 60;90.5 |
| ChPLA2-IIE (f) | GGCAGAGGGACCCCAGTGGA | 200 | 70;92 |
| ChPLA2-IIE (r) | CACGAAGCCACGGCCCTGTC | 200 | 70;92 |
| ChPLA2-V (f) | AATGCTCTCCTTGCATTGGCCATACTG | 200 | 49;90 |
| ChPLA2-V (r) | AGCTGGCAGCACTGTCTGTGCATCC | 200 | 49;90 |
| ChPLA2-X (f) | AAAAATCCTTGTCAGCTGCCGCTCGTG | 200 | 60;88 |
| ChPLA2-X (r) | TTGGGCCATCCCCTTCCTCCCAGGCCG | 200 | 60;88 |
| Beta Actine (f) | CACAGATCATGTTTGAGACCTT | 60 | 60;85 |
| Beta Actine (r) | CACAATACCAGTGGTACG | 60 | 60;85 |