Fig. 5.
The binding efficiency of plant 2C10 mAbs to PEDV. Plant-produced 2C10 IgG from N. benthamiana and lettuce were diluted 1 : 1000 before incubated in wells containing immobilized PEDV. Detection with HRP-labeled anti-human IgG antiserum yielded OD450 measurements. Data are means of the ratio of the binding of plant produced 2C10 or GST-truncated S to media of Vero cells without virus ± standard deviation (SD) of samples from three independent experiments. GST-truncated S: glutathione S -transferase fused with truncated spike protein of PEDV.