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. 2014 Sep 6;174(1):100–106. doi: 10.1016/j.vetmic.2014.08.027

Table 2.

Primers used for PIV3 detection and gene amplification.

Primers Sequences (5′–3′) Location (to NM09) Target Expected products size (bp) Annealing temperature (°C)
MF1 AGTGATCTAGATGATGATCCA 3960–3980 M 329 50
MR1 GTTATTGATCCAATTGCTGT 4269–4288
HNF GCCAATACAGAGAATGACTCATGA 7210–7233 HN 674 54
HNR CTCTTTGTGTTTTGCCWGGACA 7862–7883
NF AGGTRAGGGRGAAGARATCCT 78–98 5′-UTR-N 881 54
NR AGAGCTGCCATTCTRGTYTCAAT 936–958
LF AGAGARGTBATGGATGATCTD 11,079–11,099 L 545 54
LR GAYTCRCCTGGTTCYTGATTC 11,603–11,623
MF2 AGCATCASMARCTCYRRAATMT 3738–3759 M 1053 52
MR2 CTATTGYYTRATYTTYCCGACYCCT 4766–4790
FF1 CTCATTACCTGGTGAATTCA 4721–4740 M-F 1481 53
FR1 ACAAATGCATATCKTGGYAC 6182–6201
FF2 ACTTCRACAGTTGAYCAATA 5798–5817 F-HN 1297 53
FR2 GTCTTGTGTTTATTCCYGAYTG 7073–7094