Table 2.

Primers used for PIV3 detection and gene amplification.

Primers	Sequences (5′–3′)	Location (to NM09)	Target	Expected products size (bp)	Annealing temperature (°C)
MF1	AGTGATCTAGATGATGATCCA	3960–3980	M	329	50
MR1	GTTATTGATCCAATTGCTGT	4269–4288
HNF	GCCAATACAGAGAATGACTCATGA	7210–7233	HN	674	54
HNR	CTCTTTGTGTTTTGCCWGGACA	7862–7883
NF	AGGTRAGGGRGAAGARATCCT	78–98	5′-UTR-N	881	54
NR	AGAGCTGCCATTCTRGTYTCAAT	936–958
LF	AGAGARGTBATGGATGATCTD	11,079–11,099	L	545	54
LR	GAYTCRCCTGGTTCYTGATTC	11,603–11,623
MF2	AGCATCASMARCTCYRRAATMT	3738–3759	M	1053	52
MR2	CTATTGYYTRATYTTYCCGACYCCT	4766–4790
FF1	CTCATTACCTGGTGAATTCA	4721–4740	M-F	1481	53
FR1	ACAAATGCATATCKTGGYAC	6182–6201
FF2	ACTTCRACAGTTGAYCAATA	5798–5817	F-HN	1297	53
FR2	GTCTTGTGTTTATTCCYGAYTG	7073–7094