Fig. 5.

Flow cytometric analysis of CD and CD8 positive T cell subsets. Indicated mononuclear cells were immunostained to analyze the frequency of immune cells: (a–d) CD3⁺ cells; (e–h) CD8⁺ T cells; (i–l) CD4⁺ T cells; and (m–p) CD4⁺CD8⁺ T cells. Each bar in the graph represents geometric mean of percent of immune cells from three pigs ± SEM. Alphabet ‘a’, ‘b’ and ‘c’ represents the statistical significant difference (p < 0.05) between unvaccinated vs K-Ag, unvaccinated vs Nano-KAg, and K-Ag Vs Nano-KAg pigs, respectively.