Fig. 6.

Flow cytometry analysis of innate and regulatory T cells. Indicated mononuclear cells were immunostained to determine the frequency of immune cells: (a–d) γδ T cells; (e–h) myeloid cells; (i–k) dendritic cells rich fraction; and (l–n) Tregs. Each bar in the graph represents geometric mean of percent of immune cells from three pigs ± SEM. Alphabet ‘a’, ‘b’ and ‘c’ represents the statistical significant difference (p < 0.05) between unvaccinated vs K-Ag, unvaccinated vs Nano-KAg, and K-Ag vs Nano-KAg pigs, respectively.