Table 2.
Results of PCV2 isolation by two passages on PK-15 cells and determination of the PCV2 infectious titer in 10% (w/v) tissue homogenates from control and experimentally PCV2-infected pigs
| Experimental group | Pig no. | PIDb | Pool 1c | PCV2 (log10 TCID50/g)a |
||
| Pool 2 | Pool 3 | Pool 4 | ||||
| PCV2 | 11d | 21 | +e (4.9f) | + (4.7) | + (5.3) | + (3.7) |
| 12d | 21 | + (5.7) | + (6.1) | + (5.5) | + (4.7) | |
| 13d | 25 | + (4.5) | + (5.7) | + (5.5) | + (3.9) | |
| 14 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) | |
| 15 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) | |
| PCV2+immunisation | 16 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) |
| 17 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) | |
| 18d | 22 | + (5.3) | + (5.9) | + (4.9) | + (2.7) | |
| 19 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) | |
| 20 | 36 | + (<1.5) | + (<1.5) | + (<1.5) | + (<1.5) | |
a
log10TCID50/g=50% tissue culture infectious dose per gram of tissue.
b
Postinoculation day.
c
Pool 1: tissue homogenate of inguinal and prescapular lymph nodes; pool 2: tissue homogenate of spleen, mesenteric and bronchial lymph nodes; pool 3: tissue homogenate of lung, liver, and kidney; pool 4: tissue homogenate of tonsils.
d
Pig that died or was euthanized before termination of the experiment on PID 36.
e
Plus (+) denotes isolation of PCV2.
f
The infectious titer of PCV2.