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. 1998 Dec 10;64(1):7–22. doi: 10.1016/S0378-1135(98)00254-5

Fig. 1.

Fig. 1

Specificity of the test on EU-type (111/92) and US-type (VR2332) reference viruses. RNA was extracted from 111/92 stock, VR2332 stock and water, followed by reverse transcription with a cocktail of 3 RT primers designed to prime cDNA synthesis from ORFs 5 and 7 of all known types of PRRSV. cDNA aliquots were examined by singleplex PCR with primers specific for all known PRRSV-types (ORF 7 PCR) or specific for EU- or US-type PRRSV (EU ORF 5 or US ORF 5 PCR, respectively). Lane 1, Phi-X HaeIII digest. Lanes 2–4, ORF 7 PCR on the following templates: 2, 111/92. 3, VR2332. 4, H2O. Lanes 5–7, EU ORF 5 PCR on the following templates: 5, 111/92. 6, VR2332. 7, H2O. Lanes 8–10, US ORF 5 PCR on the following templates: 8, 111/92. 9, VR2332. 10, H2O.