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. 1998 Dec 10;64(1):7–22. doi: 10.1016/S0378-1135(98)00254-5

Fig. 2.

Fig. 2

Typing of Danish field PRRSV isolates by ORF 7 amplicon size. ORF 7 RT-PCR reactions from 4 MAb-typed Danish field PRRSV isolates (part of the dataset presented in Table 2) were examined by highly sieving agarose gel electrophoresis. To facilitate the precise comparison of ORF 7 amplicon size, a marker (`5 + 7 marker') containing pooled RT-PCR product from ORF 5 and ORF 7 PCR on US- (VR2332) and EU- (111/92) type virus was utilized in lanes across the gel. The 4 bands in the 5 + 7 marker are: 818 bp (US ORF 5), 719 bp (DK ORF 5), 660 bp (ORF 7 from US-type virus), 637 bp (ORF 7 from EU-type virus). Lanes 1, 4 and 7, 5 + 7 marker. Lanes 2 and 3, two isolates from 1997 MAb-typed as American-type. Lanes 5 and 6, two isolates from 1993 and 1995 MAb-typed as European-type.