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. 1998 Dec 10;64(1):7–22. doi: 10.1016/S0378-1135(98)00254-5

Fig. 3.

Fig. 3

Sensitivity of the test on EU-type (111/92) and US-type (VR2332) reference viruses. The two reference viruses were diluted in whole (undiluted and unfractionated) boar semen to the concentrations given below. 30 μl of each dilution was examined by RT-PCR. Lane 1, Phi-X HaeIII marker. Lanes 2–5, ORF 7 PCR on the following dilutions of 111/92 (EU-type virus): (2) 10 TCID50 ml−1. (3) 1 TCID50 ml−1. (4) 0.1 TCID50 ml−1. (5) 0.01 TCID50 ml−1. Lanes 6–9, ORF 7 PCR on the following dilutions of VR2332 (US-type virus): (6) 100 TCID50 ml−1. (7) 10 TCID50 ml−1. (8) 1 TCID50 ml−1. (9) 0.1 TCID50 ml−1. Lane 10, negative control: ORF 7 PCR on boar semen without PRRSV. Lanes 11–14, EU ORF 5 PCR on the following dilutions of 111/92 (EU-type virus): 11, 10 TCID50 ml−1. 12, 1 TCID50 ml−1. 13, 0,1 TCID50 ml−1. 14, 0,01 TCID50 ml−1. Lane 15, negative control: EU ORF 5 PCR on 100 TCID50 ml−1 of VR2332 (US-type virus). Lanes 16–19, US ORF 5 PCR on the following dilutions of VR2332 (US-type virus): 16, 100 TCID50 ml−1. 17, 10 TCID50 ml−1. 18, 1 TCID50 ml−1. 19, 0,1 TCID50 ml−1. Lane 20, negative control: US ORF 5 PCR on 10 TCID50 ml−1 111/92 (DK-type virus).