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. 1998 Dec 10;64(1):7–22. doi: 10.1016/S0378-1135(98)00254-5

Fig. 4.

Fig. 4

Examination of mixed-type PRRSV populations by ORF 7 PCR. Reference viruses VR2332 (American-type) and 111/92 (European-type) were mixed in different ratios (based on TCID50), but maintaining a constant PRRSV concentration of 100 TCID50 ml−1 (thus ensuring that even a 3% minority-type would be above the detection sensitivity of the RT-PCR), and the mixed populations were analyzed by ORF 7 RT-PCR. Lanes 1 and 7, `5 + 7' size marker, as described in Fig. 2. Lane 2, EU-type virus only. Lane 3, EU : US-type ratio of 29 : 1. Lane 4, EU : US-type ratio of 1 : 1. Lane 5, EU : US-type ratio of 1 : 29. Lane 6, US-type virus only.