Table 1.

BuCoV detection in buffalo calves with diarrhoea by virus isolation, conventional and real-time RT-PCR.

Prot. no.	Age of the animals	Sample type	Virus isolation	RT-PCR	Real-time RT-PCRa
339/07-A	15 days	Faeces	ND	+	2.45 × 104
339/07-B	11 days	Faeces	ND	+	6.54 × 103
339/07-C	11 days	Faeces	+	+	8.17 × 104
339/07-D	10 days	Faeces	ND	+	3.31 × 102
339/07-E	11 days	Faeces	ND	+	5.08 × 103
339/07-F	7 days	Faeces	ND	+	1.13 × 104
82/08	23 days	Intestinal content	−	+	−
153/08-A	15 days	Intestinal content	ND	+	3.26 × 105
153/08-B	18 days	Intestinal content	+	+	7.16 × 106
155/08	20 days	Intestinal content	+	+	4.30 × 106

+, positive; −, negative; ND, not done.

^aReal-time RT-PCR results are expressed as RNA copies μl⁻¹ of template.