Fig. 1.

Histological lesions of small intestines and canine bocavirus 2 (CaBoV-2) in situ hybridization. (A) Prominent histological lesions comprised atrophied, shortened and fused villi (asterisks), loss of enterocytes (arrow heads) and large crypt regenerates (arrows) in the small intestine (HE, 200×). (B and C) Within the submucosal lymphoid tissue (asterisks) CaBoV-2 nucleic acid was demonstrated by in situ hybridization ((B) HE, 400×); (C) in situ hybridization with CaBoV-2-specific probes, 400×; (D) Nearly diffuse positive staining of enterocyte nuclei (arrow) by in situ hybridization with CaBoV-2-specific probes in the small intestine of a dog with presumed minute virus of canines infection (400×). (For interpretation of the references to color near the citation of this figure, the reader is referred to the web version of the article.)