. 2014 Mar 27;171(1):74–86. doi: 10.1016/j.vetmic.2014.03.017

Table 1.

The first animal experiment design.

	Group and pig numbers	Inculation and numbers of segments per pig	Time of virus infection
Intestinal ligation	I (n1 = 5)	PBS (1 ml per segment, n2 = 3) PBS (1 ml per segment, n2 = 3) SHXB (1 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per segment, n2 = 3) SHXB (1 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per segment, n2 = 3)	3 h (From the first injection to sacrifice) 15 min (from the second injection to sacrifice) 3 h (from the first injection to sacrifice) 15 min (from the second injection to sacrifice)
Intestinal ligation	II (n1 = 5)	PBS (1 ml per segment, n2 = 3) PBS (1 ml per segment, n2 = 3) STC3 (1 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per segment, n2 = 3) STC3 (1 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per segment, n2 = 3)	3 h (From the first injection to sacrifice) 15 min (from the second injection to sacrifice) 3 h (from the first injection to sacrifice) 15 min (from the second injection to sacrifice)
Oral administ-ration	III (n1 = 5)	SHXB (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig)	48 h (From administration to sacrifice)
Oral administ-ration	IV (n1 = 5)	STC3 (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig)	48 h (from administration to sacrifice)