. 2014 Mar 27;171(1):74–86. doi: 10.1016/j.vetmic.2014.03.017

Table 2.

The second animal experiment design.

Group and pig numbers	Oral administration and time of infection	Inculation and numbers of segments per pig	Time of HI-E. coli inoculation
I (n1 = 5)	PBS (20 ml, 48 h)	PBS (1 ml per segment, n2 = 3) PBS (1 ml per segment, n2 = 3)	3 h (From the first injection to sacrifice) 15 min (From the second injection to sacrifice)
II (n1 = 5)	PBS (20 ml, 48 h)	HI-E. coli (1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	3 h (From the first injection to sacrifice)
II (n1 = 5)	PBS (20 ml, 48 h)	HI-E. coli (1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	15 min (From the second injection to sacrifice)
III (n1 = 5)	SHXB (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig, 48 h)	HI-E. coli (1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	3 h (From the first injection to sacrifice)
III (n1 = 5)	SHXB (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig, 48 h)	HI-E. coli (1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	15 min (From the second injection to sacrifice)
IV (n1 = 5)	STC3 (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig, 48 h)	HI-E. coli(1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	3 h (From the first injection to sacrifice)
IV (n1 = 5)	STC3 (20 ml of 1 × 10⁷ TCID₅₀ ml⁻¹ per pig, 48 h)	HI-E. coli(1 ml of 1 × 10⁸ cfu ml⁻¹ per segment, n2 = 3)	15 min (From the second injection to sacrifice)