. 2016 Feb 9;186:90–96. doi: 10.1016/j.vetmic.2016.02.004

Table 1.

Summary of virus-serum neutralizing antibody and serum IgG ELISA antibody.

Serum^A	Virus-serum neutralizing assay^B		Serum IgG ELISA^C
Serum^A	PEDV	PdCV	PEDV	PdCV
PEDV 1	256^a	<20	5120^a	80^a
PEDV 2	230^a	<20	5120^a	40^a
PEDV 3	256^a	<20	5120^a	80^a
PEDV 4	256^a	<20	5120^a	80^a
PdCV 1	<20	382^a	40^b	10240^b
PdCV 2	<20	544^a	40^b	10240^b
PdCV 3	<20	544^a	80^b	10240^b
PdCV 4	<20	544^a	80^b	10240^b
Control 1	<20	<20	<20	<20
Control 2	<20	<20	<20	<20
Control 3	<20	<20	<20	<20
Control 4	<20	<20	<20	<20

Gn piglets were immunized intramuscularly twice (two weeks apart) with 0.3 mg of inactivated PEDV or PdCV antigen. For antigen preparation, 1.2 mg of purified PEDV and PdCV stocks were inactivated by 0.05% formalin, and mixed with 3 ml of Alhydrogel aluminum Hydroxide Gel Adjuvant in 1 ml of PBS. The control piglets were immunized with 1 ml of adjuvant alone. Sera were collected from PEDV antigen, PdCV antigen, or mocked-inoculated Gn piglets at week 4 post-immunization and were heat inactivated at 56 °C for 30 min.

Virus-serum neutralizing antibody titer was determined by a plaque reduction neutralization assay using PEDV or PdCV. Data are average of three independent VN assay. Value within a column followed by the different lowercase letters (a and b) are significantly different (P < 0.05).

Serum IgG titer was determined by ELISA using purified PEDV or PdCV as coated antigens.