Table 2.
Summary of immunohistochemical (IHC) staining of intestinal tissues.
| Pig number | Anti-PEDV serum |
Anti-PdCV serum |
||||||
|---|---|---|---|---|---|---|---|---|
| Duodenum | Jejunum | Ileum | Colon | Duodenum | Jejunum | Ileum | Colon | |
| PEDV I | 4 | 4 | 4 | 2 | 0 | 0 | 0 | 0 |
| PEDV II | 4 | 4 | 4 | 1 | 0 | 0 | 0 | 0 |
| PEDV III | 4 | 4 | 4 | 2 | 0 | 0 | 0 | 0 |
| PEDV IV | 4 | 4 | 4 | 2 | 0 | 0 | 0 | 0 |
| PdCV I | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 0 |
| PdCV II | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 0 |
| PdCV III | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 0 |
| PdCV IV | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 0 |
| Control I | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control II | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control III | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Control IV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Note: IHC staining magnitude was scored for each piglet. 0: no antigen; 1: mild, less than 20% of epithelial cells were positive for antigen; 2: mild to moderate, 20–50% of epithelial cells were positive; 3: moderate, 50–80% of epithelial cells were positive; 4: severe, more than 80% epithelial cells were positive. No antigens were detected for PEDV or PdCV-infected intestinal tissues using control serum.