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Overlap of the LAMP primer sets on the PF3D7_0112300 18S rRNA gene. The new forward primer begins after the initial 11 nucleotides of the Poon et al.’s forward primer. The reverse primer Pf-B3 are identical. The other new primers, which are underlined, are shifted from the Poon et al.’s corresponding primers by 4–10 nucleotides.
