Table 2.
Comparative detection of BVDV infected samples by different tests
| BVDV strain | Sample dilutions |
|||||||||||||||
| Neat | 10−1 | 10−2 | 10−3 | 10−4 | 10−5 | 10−6 | 10−7 | |||||||||
| NADL | ||||||||||||||||
| RT–PCR | + | + | + | + | + | − | − | − | ||||||||
| Two-tube TaqMan/RT−PCR | + | + | + | + | + | − | − | − | ||||||||
| One-tube TaqMan/RT–PCR | + | + | + | + | + | Wa+ | − | − | ||||||||
| Type II-125 | ||||||||||||||||
| RT–PCR | + | + | + | + | + | − | − | − | ||||||||
| Two-tube TaqMan/RT–PCR | + | + | + | + | + | − | − | − | ||||||||
| One-tube TaqMan/RT–PCR | + | + | + | + | + | + | − | − | ||||||||
| pi BVDV + serum | ||||||||||||||||
| RT–PCR | + | − | − | − | − | NAb | − | − | ||||||||
| Two-tube TaqMan/RT–PCR | + | − | − | − | − | NA | − | − | ||||||||
| One-tube TaqMan/RT–PCR | + | + | + | − | − | NA | − | − | ||||||||
a
W: weak.
b
NA: not available.