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. 2010 Sep 19;117(1):23–30. doi: 10.1016/j.actatropica.2010.09.004

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Copyright © 2010 Elsevier B.V. All rights reserved.

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Fig. 2 — Cysts of Acanthamoeba isolates grew in xenic (top row) and axenic (bottom row) culture conditions. In xenic culture condition, cysts of respective isolates revealed the typical cysts morphology of the named species (A) A. castellanii (IMU9), (B) A. culbertsoni (IMU11), (C) A. griffin (IMU14), (D) A. lenticulata (IMU17), (E) A. polyphaga (IMU19). In contrast, axenic cysts of all isolates no longer revealed the characteristic morphologies of the respective species; they were generally bigger and round in shapes. Images were viewed under bright field microscopy. Bars represent 10 μm.