Table 1.
Percentage of sequence similarities for present Acanthamoeba isolates against the published 18S rRNA gene sequences of specified Acanthamoeba spp. retrieved from the GenBank database.
| ASA.S1 sequences of Acanthamoeba isolates | Published 18S rRNA sequences of Acanthamoeba spp. |
|
|---|---|---|
| Most matched (%) | Other closely matched (%) | |
| IMU1, IMU2, IMU6 and IMU7 | A. castellanii CDC:0184:V014 (99) |
A. polyphaga from a pond in Germany (97) A. quina ATCC 50241 (97) |
| IMU3 | A. castellanii CDC:0184:V014 (99) |
A. polyphaga from a pond in Germany (97) A. culbertsoni Diamond (97) A. rhysodes 7AR (97) A. lugdunensis 312-2 (97) |
| IMU4 and IMU5 | A. castellanii Castellanii (99) |
A. polyphaga HC-2 (99) A. rhysodes BCM:0685:116 (99) A. culbertsoni Diamond (99) A. lugdunensis 312-2 (99) |
| IMU8 | A. castellanii CDC:0180:1 (99) |
A. culbertsoni Diamond (99) A. hatchetti 3ST (99) A. polyphaga ATCC 50372 (99) |
| IMU9 | A. castellanii CDC:0786:V042 (100) |
A. polyphaga HC-2 (99) A. rhysodes BCM:0685:116 (99) A. culbertsoni Diamond (99) A. lugdunensis 312-2 (99) A. triangularis SH621 (98) |
| IMU10, IMU11, IMU12 and IMU13 | A. culbertsoni Diamond (100) |
A. castellanii CDC:0180:1 (99) A. hatchetti 3ST (99) A. polyphaga ATCC 50372 (99) |
| IMU14 | A. griffin H37 (100) |
A. griffin S-7 (98) A. polyphaga Panola Mountain (98) A. pearcei ATCC 50435 (96) |
| IMU15 | A. hatchetti 3ST (99) |
A. culbertsoni Diamond (99) A. polyphaga ATCC 50372 (99) A. castellanii ATCC 50370 (99) |
| IMU16 and IMU17 | A. lenticulata Jc-1, CRIB56, 72/2, 7327, 45 (97) | Nil |
| IMU18 | A. triangularis SH621 (99) |
A. polyphaga ATCC 30461 (99) A. castellanii ATCC 30010 (98) A. culbertsoni Diamond (98) A. hatchetti 3ST (98) A. rhysodes 7AR (98) A. lugdunensis 312-2 (98) |
| IMU19, IMU20 and IMU21 | A. quina ATCC 50241 (99) |
A. polyphaga 5SU (99) A. palestinensis 36KL (99) A. castellanii Castellanii (98) A. hatchetti 3ST (98) |
GenBank accession numbers: A. castellanii: ATCC 30010 (EF554328), ATCC 50370 (AF534136), Castellanii (U07413), CDC:0180:1 (U07405), and CDC:0786:V042 (U07403); A. lenticulata: CRIB56 (EU377584) and 7327 (U94731); A. lugdunensis: 312-2 (AF260718); A. palestinensis 36KL (AF260719); A. pearcei ATCC 50435 (AF019053); A. polyphaga: 5SU (AF260725), ATCC30461 (AY026243), ATCC 50372 (AF534138), HC-2 (AF019056) and Panola Mountain (AF019052); A. rhysodes: 7AR (AF260720) and BCM:0685:116 (U07406). Others are listed in Fig. 1.