Table 1.

Details of FCoV primers and probe

Oligos	Size (nt)	TTm (°C)	G+C%	Priming site	Prod. size (nt)	Sequence 5′→3′
FCoV
No.66 (F)	18	50	39	2720→2737		CTG CAT GTC AAA CTA TTG
No.67 (R)	17	52	53	3521←3504		CTT GTG CAT CAG CAC TC
No.30 (R)	16	48	50	2923←2981		TGC TAT TAT GGG ACG G
No.66 (F) + No.67 (R)					800
No.66 (F) + No.30 (R)					217
Probe
FCoV-S	18	66	50	2816→2834		ATT GGC ATC TGT TGA GGC

nt = nucleotides.

TTm = theoretical annealing temperature based on 100% identity.

F = forward primer; R = reverse primer.