Fig. 1.

Characterization of the PEDV antigens. (A) Electron micrograph of the purified PEDV whole virus. Negatively-stained PEDV particles showing pleomorphism are labeled with black arrows whereas the “regular” virions (rounded or elongated shaped) are marked by white arrows. The bar represents 100 nm. (B) Western blot analysis of recombinant PEDV Ac, E and ORF3C peptides. Expression of PEDV Ac, E and ORF3C peptides in bacteria was confirmed with an anti-His monoclonal antibody (MAb). Approximately 150 ng of each protein were separated by SDS-PAGE and subjected to western blot. (C) SDS-PAGE and western blot analysis of recombinant PEDV Nsp1, ADRP and Nsp2 peptides. Expression of PEDV Nsp1, ADRP and Nsp2 peptides was identified using SDS-PAGE (left panel) and confirmed with an anti-His MAb (right panel). Approximately 100 ng of each protein were separated by SDS-PAGE and subjected to western blot. An HRP-conjugated goat anti-mouse IgG was used as the secondary antibody. (D) SDS-PAGE of the recombinant PEDV S1 peptide. The arrowheads indicate bands of the target proteins.