Fig. 2.

Immunofluorescence assay (IFA) of PEDV-infected Vero cells. Vero cells were infected with PEDV and stained with rabbit anti-PEDV-Ac (A, B), anti-PEDV-ORF3C (C, D), or anti-PEDV-Nsp2 (E, F) antibodies at 48 h post-infection. A mouse anti-S1 monoclonal antibody (G, H) and pre-immunization rabbit serum (I, J) were used as positive and negative controls, respectively. AlexaFluor 488-conjugated goat anti-rabbit IgG and goat anti-mouse IgG (green) were used as secondary antibodies, as appropriate. Antibody staining merged with DAPI nuclear staining (blue) is shown; magnification = 20× (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).