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Detection of antibodies against PEDV peptides in porcine sera by western blot. Representative serum samples from diarrheic pigs were used in western blots to detect PEDV S1 (A), Ac (B), ORF3C (C), and E proteins (D); 5 μg of recombinant peptides were loaded in each lane. HRP-conjugated goat anti-porcine IgG was used as the secondary antibody, and PEDV-negative serum was used as negative control.
