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. 2019 Oct 12;240:108449. doi: 10.1016/j.vetmic.2019.108449

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Fig. 1 — Expression, purification, and identification of BVDV NS5B recombinant protein and detection of serum anti-NS5B antibody titer. (A) The expression and purification of NS5B recombinant protein were analyzed by SDS-PAGE. Lane 1: pET28a vector control; lane 2: induction with 1 mM IPTG; lane 3: soluble protein in supernatant after sonication; lane 4: inclusion body in precipitate after sonication; lane 5: purified NS5B protein; M: molecular weight markers, size indicated in kDa. (B) Antigenic analysis of Western blotting, lanes 1–5: same as (A); samples were reacted with anti-His antibodies. (C) Determination of serum antibody titers after immunization by iELISA.